… Indeed, it is a realistic possibility that the complete genome of a filamentous fungus will
be determined at the nucleotide level, with Aspergillus nidulans as the strongest …

… Aspergillus nidulans has been one of the most widely studied species in terms of
genetics and biochemistry [9, 10]. It is often used as model organism in the identification of …

… /Cas) developed in filamentous fungi and RNA … gene manipulation methods in filamentous
fungi and presents the molecular tools available to researchers investigating filamentous fungi…

… -free gene editing and multiple gene editing in filamentous fungi. … for efficient gene
deletion/integration and was used in … into the cell is a rapid method to disrupt a gene, which may …

… groups of fungi can be transformed, and the genetic manipulation of … In this chapter, we focus
on five filamentous fungi comprising … or agriculture, ie Aspergillus niger, Aspergillus oryzae, …

… Filamentous fungi, and in particular Aspergillus nidulans and … for eukaryotic gene organization
and gene regulation. These … , and the methodology for classical genetic analysis is highly …

… Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic
… For example, while molecular genetic tools have been developed, including CRISPR/Cas9…

… the initial framework to adapt this gene editing technology for filamentous fungi. In this review,
… for different filamentous fungi is presented, including integration of the CAS9 gene into the …

… , which contains a fungal expression cassette with the Aspergillus nidulans gpdA promoter
(Punt et al. 1992), a multiple cloning site (MCS), and the A. nidulans trpC terminator [trpC(t)] (…

… for Aspergillus nidulans can be used for CRISPR based gene editing in filamentous fungi. We
… Even model fungi, where efficient gene targeting has been implemented via eg elimination …